Identification of novel sesterterpenes by genome mining of phytopathogenic fungi Phoma and Colletotrichum sp. Issue 12 (21st March 2018)

• Record Type: Journal Article
• Authors: Gao, Lei; Narita, Koji; Ozaki, Taro; Kumakura, Naoyoshi; Gan, Pamela; Minami, Atsushi; Liu, Chengwei; Lei, Xiaoguang; Shirasu, Ken; Oikawa, Hideaki
• Is Part Of: Tetrahedron letters. Volume 59:Issue 12(2018)
• Page Start: 1136
• Page End: 1139
• Publication Date: 2018-03-21
• Subjects: Sesterterpene synthase -- Genome mining -- Natural product biosynthesis -- Phytopathogen -- Heterologous expression; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tetlet.2018.02.022
• Languages: English
• ISSNs: 0040-4039

Abstract

Graphical abstract: Highlights: Heterologus production of seven novel fungal terpenes. Genome mining of fungal sesterterpenes. Cryptic gene clusters expressed during the infection by Colletotrichum orbiculare. Abstract: Two homologous gene clusters for the biosynthesis of sesterterpenes betaestacins were identified from two phytopathogens, Phoma betae and Colletotrichum orbiculare . Heterologous expression of identified oxidation enzymes with previously-characterized PbTS1 (BtcAPb ) resulted in the production of seven novel sesterterpenes. Although both strains possessed homologous enzymes, oxidation state of corresponding products were different from each other, suggesting that structural diversification of sesterterpene skeletons might be achieved by these homologous enzymes with different functions.