Assessment of mutagenic, recombinogenic and carcinogenic potential of titanium dioxide nanocristals in somatic cells of Drosophila melanogaster. (February 2018)

• Record Type: Journal Article
• Authors: Carvalho Naves, Maria Paula; de Morais, Cássio Resende; Silva, Anielle Christine Almeida; Dantas, Noelio Oliveira; Spanó, Mário Antônio; de Rezende, Alexandre Azenha Alves
• Is Part Of: Food and chemical toxicology. Volume 112(2018)
• Page Start: 273
• Page End: 281
• Publication Date: 2018-02
• Subjects: SMART -- Somatic mutation and recombination test -- Test for detection of epithelial tumor clones -- WTS -- Warts -- Nanotechnology; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.12.040
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Nanoparticles have been widely used in several sectors and their long-term effect on the body and environment remains unknown. To evaluate the mutagenic, recombinogenic and carcinogenic potential of 11 nm titanium dioxide nanocrystals (TiO2 NCs), the Somatic Mutation and Recombination Test (SMART) and the Test for Detection of Epithelial Tumors Clones (Warts-Wts) were used, both in Drosophila melanogaster. Third-instar larvae (72 + 4 h), obtained in both tests, were treated with different concentrations of TiO2 NCs ranging from 6.25 to 100 mM. Ultrapure water and urethane were used as negative and positive controls, respectively. At ST cross, all concentrations of TiO2 NCs showed a significant increase in the frequencies of mutant spots, demonstrating higher recombination rates. At the HB cross, only the 50 mM concentration showed a negative result. In the Wts Test, all used concentrations were carcinogenic, except for the 100 mM one, which was toxic. No relationship was demonstrated between the used concentrations and the obtained responses. There was no interference of the cytochrome P450 enzyme complex in the induction of mutant spots. Highlights: The mutagenic/recombinogenic potential of TiO2 NCs (11 nm) was studied in vivo. The carcinogenic potential of TiO2 NCs (11 nm) was studied in vivo. Progenie of Wts is more sensitive to TiO2 NCs (11 nm) than SMART progenie. TiO2 NCs (11 nm) has mutagenic and recombinogenic action in ST and HB crosses. TiO2 NCs (11 nm) has carcinogenic action.