Long Non-coding RNA BC168687 is Involved in TRPV1-mediated Diabetic Neuropathic Pain in Rats. (15th March 2018)

• Record Type: Journal Article
• Authors: Liu, Chenglong; Li, Congcong; Deng, Zeyu; Du, Errong; Xu, Changshui
• Is Part Of: Neuroscience. Volume 374(2018)
• Page Start: 214
• Page End: 222
• Publication Date: 2018-03-15
• Subjects: DNP diabetic neuropathic pain -- DRG dorsal root ganglia -- FPG fasting plasma glucose -- lncRNAs long noncoding RNAs -- MAPK mitogen-activated protein kinase -- MWT mechanical withdrawal thresholds -- PBS phosphate buffer saline -- PNS peripheral nervous system -- SGCs satellite glial cells -- siRNA small interfering RNA -- STZ streptozotocin -- TNF tumor necrosis factor -- TRPV1 transient receptor potential vanilloid type 1 -- TWL thermal withdrawal latencies; diabetic neuropathic pain -- long non-coding RNAs -- DRG -- TRPV1; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2018.01.049
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Long non-coding RNA BC168687 is involved in diabetic neuropathic pain in rats. LncRNA BC168687 siRNA may reduce expression of TRPV1 receptors in DRG of DNP rats. ERK and p38 signaling pathways might be regulated by lncRNA BC168687 siRNA in diabetic neuropathic pain. Abstract: Long noncoding RNAs (lncRNAs) participate in a diverse range of molecular and biological processes, and dysregulation of lncRNAs has been observed in the pathogenesis of various human diseases. We observed alterations in mechanical withdrawal thresholds (MWT) and thermal withdrawal latencies (TWL) in streptozotocin (STZ)- induced diabetic rats treated with small interfering RNA (siRNA) of lncRNA BC168687. We detected expression of transient receptor potential vanilloid type 1 (TRPV1) in rat dorsal root ganglia (DRG) by a series of molecular experiments. We determined relative levels of tumor necrosis factor (TNF)- α and interleukin (IL)- 1β in rat serum by enzyme-linked immunosorbent assay (ELISA). In addition, we examined extracellular regulated protein kinases (ERK) and p38 mitogen- activated protein kinase (MAPK) signaling pathways by Western blot (WB). We showed that the MWT and TWL of diabetic rats increased significantly compared with control. Expression of TRPV1 receptors in DRG substantially decreased. Relative levels of TNF- α and IL- 1β in the serum of lncRNA BC168687 siRNA-treated rats were reduced. Phosphorylation (p)- ERK and p- p38 signaling pathways in DRG were also decreased. Taken together, we concluded lncRNA BC168687 siRNA may alleviate TRPV1- mediated diabetic neuropathic pain.