MiR-221-3p Inhibits Schwann Cell Myelination. (21st May 2018)

• Record Type: Journal Article
• Authors: Zhao, Lili; Yuan, Ying; Li, Ping; Pan, Jiacheng; Qin, Jing; Liu, Yisheng; Zhang, Yu; Tian, Feng; Yu, Bin; Zhou, Songlin
• Is Part Of: Neuroscience. Volume 379(2018)
• Page Start: 239
• Page End: 245
• Publication Date: 2018-05-21
• Subjects: DMEM Dulbecco's modified Eagle's medium -- MBP Myelin basic protein -- Nab1 NGF1-A binding protein 1 -- NGF nerve growth factor -- PNS peripheral nervous system -- SCs Schwann Cells; miR-221-3p -- Schwann Cell -- myelination -- Nab1 -- peripheral nerve injury; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2018.03.019
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: miR-221-3p could inhibit the myelination of Schwann Cells when co-cultured with dorsal root ganglion cells in vitro . miR-221-3p could downregulate NGF1-A binding protein 1 (Nab1) which was essential for Schwann Cells myelination. The promotion of miR-221-3p antagomir on Schwann Cell myelination could be reversed by suppressing the expression of Nab1. Abstract: Following peripheral nerve injury, Schwann Cells (SCs) undergo dedifferentiation, proliferation, migration, and remyelination. Recent works demonstrated the importance of the short non-coding RNA (miRNAs) in SC dedifferentiation and remyelination after nerve injury. Previously, we found some miRNAs like miR-9, miR-221, miR-222 and miR-182 could regulate the proliferation and migration of SCs. Therefore, it is imperative to ask whether these miRNAs could regulate the myelination of SCs. Here we demonstrated that miR-221-3p could inhibit the myelination of SCs when co-cultured with dorsal root ganglion cells in vitro . In addition, NGF1-A binding protein 1 (Nab1) which was essential for SCs myelination could be downregulated by miR-221-3p . Suppressing the expression of Nab1 could reverse the promotion of miR-221-3p antagomir on SC myelination. The effects of miR-221-3p on SC myelination might be used to improve peripheral nerve regeneration, thus offering a new approach to peripheral nerve repair.