Ecotoxicological effects of the herbicide glyphosate in non-target aquatic species: Transcriptional responses in the mussel Mytilus galloprovincialis. (June 2018)

• Record Type: Journal Article
• Authors: Milan, M.; Dalla Rovere, G.; Smits, M.; Ferraresso, S.; Pastore, P.; Marin, M.G.; Bogialli, S.; Patarnello, T.; Bargelloni, L.; Matozzo, V.
• Is Part Of: Environmental pollution. Volume 237(2018)
• Page Start: 442
• Page End: 451
• Publication Date: 2018-06
• Subjects: Glyphosate -- Herbicide -- Mytilus galloprovincialis -- Bivalve -- Transcriptomics; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2018.02.049
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Glyphosate has been the most widely used herbicide worldwide over the last three decades, raising increasing concerns for its potential impacts on environmental and human health. Recent studies revealed that glyphosate occurs in soil, surface water, and groundwater, and residues are found at all levels of the food chain, such as drinking water, plants, animals, and even in humans. While research has demonstrated that glyphosate can induce a broad range of biological effects in exposed organisms, the global molecular mechanisms of action still need to be elucidated, in particular for marine species. In this study, we characterized for the first time the molecular mechanisms of action of glyphosate in a marine bivalve species after exposure to environmentally realistic concentrations. To reach such a goal, Mediterranean mussels Mytilus galloprovincialis, an ecologically and economically relevant species, were exposed for 21 days to 10, 100, and 1000 μg/L and digestive gland transcriptional profiles were investigated through RNA-seq. Differential expression analysis identified a total of 111, 124, and 211 differentially regulated transcripts at glyphosate concentrations of 10, 100, and 1000 μg/L, respectively. Five genes were found consistently differentially expressed at all investigated concentrations, including SERP2, which plays a role in the protection of unfolded target proteins against degradation, the antiapoptotic protein GIMAP5, and MTMR14, which is involved in macroautophagy. Functional analysis of differentially expressed genes reveals the disruption of several key biological processes, such as energy metabolism and Ca 2+ homeostasis, cell signalling, and endoplasmic reticulum stress response. Together, the results obtained suggest that the presence of glyphosate in the marine ecosystem should raise particular concern because of its significant effects even at the lowest concentration. Graphical abstract: Image 1 Highlights: Ecotoxicological effects of glyphosate were investigated in mussels. Environmentally realistic concentrations determined transcriptional effects in mussels. Glyphosate determines transcriptional changes of genes involved in key cell functions. Glyphosate disrupts energy metabolism, Ca2+ homeostasis, cell signalling. Abstract : Mussel transcriptional responses to glyphosate.