Complex assembly, crystallization and preliminary X‐ray crystallographic analysis of the chicken CD8αα–BF2*0401 complex. Issue 9 (1st September 2014)

• Record Type: Journal Article
• Authors: Liu, Yanjie; Chen, Rong; Tariq, Mansoor; Xia, Chun
• Is Part Of: Acta crystallographica. Volume 70:Issue 9(2014:Sep.)
• Page Start: 1264
• Page End: 1267
• Publication Date: 2014-09-01
• Subjects: chicken -- CD8αα -- BF2*0401; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X14017154
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : In the process of antigen presentation, the MHCI–CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X‐ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα–BF2*0401) are reported. This complex was verified by SDS–PAGE analysis of a CD8αα–BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, β 2 ‐microglobulin and CD8α, respectively. The crystal of CD8αα–BF2*0401 diffracted to 2.8 Å resolution and belonged to space group P 21, with unit‐cell parameters a = 90.6, b = 90.8, c = 94.9 Å, β = 98°. The Matthews coefficient and solvent content were calculated to be 2.88 Å 3 Da −1 and ∼57.3%, respectively.