An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection. Issue 26 (15th June 2015)

• Record Type: Journal Article
• Authors: Mayorga-Martinez, Carmen C.; Chamorro-García, Alejandro; Serrano, Lorena; Rivas, Lourdes; Quesada-Gonzalez, Daniel; Altet, Laura; Francino, Olga; Sánchez, Armand; Merkoçi, Arben
• Is Part Of: Journal of materials chemistry. Volume 3:Issue 26(2015)
• Page Start: 5166
• Page End: 5171
• Publication Date: 2015-06-15
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c5tb00545k
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A novel impedimetric label-free genosensor for highly sensitive DNA detection using a sensing platform based on thionine and iridium oxide nanoparticles. Abstract : An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.