An Azo Coupling Strategy for Protein 3‐Nitrotyrosine Derivatization. Issue 48 (1st August 2019)

• Record Type: Journal Article
• Authors: Liu, Yuxin; Zhou, Pengcheng; Da, Honghong; Jia, Huiyi; Bai, Feifei; Hu, Guodong; Zhang, Baoxin; Fang, Jianguo
• Is Part Of: Chemistry. Volume 25:Issue 48(2019)
• Page Start: 11228
• Page End: 11232
• Publication Date: 2019-08-01
• Subjects: azo coupling -- bioorthogonal reactions -- fluorescence -- 3-nitrotyrosine -- protein modifications; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201901828
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Herein, a strategy for the selective derivatization of 3‐nitrotyrosine‐containing proteins using the classic azo coupling reaction as the key step is described. This novel approach featured multiple advantages and was successfully applied to detect picomole levels of protein tyrosine nitration in biological samples. Abstract : Fluorescence : An azo coupling strategy for the selective derivatization of 3‐NT‐containing proteins is presented. This strategy allows the specific introduction of a bioorthogonal group to 3‐NT for the detection and enrichment of 3‐NT‐containing proteins, which facilitates the study of the functions of PTN under physiological and pathological conditions.