A simple fabricated microfluidic chip for urine sample-based bladder cancer detection. (3rd October 2018)

• Record Type: Journal Article
• Authors: Geng, Chunyang; Li, Chiyu; Li, Wang; Yan, Wo; Li, Jing; Aziz, Aziz Ur Rehman; Zhai, Xiaofeng; Sun, Weibing; Liu, Bo
• Is Part Of: Journal of micromechanics and microengineering. Volume 28:Number 11(2018:Nov.)
• Publication Date: 2018-10-03
• Subjects: bladder cancer -- microfluidic chip -- cancer detection; Microelectromechanical systems -- Periodicals; Micromechanics -- Periodicals; 621.38105
• DOI: 10.1088/1361-6439/aae016
• Languages: English
• ISSNs: 0960-1317

Abstract

Abstract: Bladder cancer is the most common malignancy of the urinary system with high morbidity and worldwide mortality. However, there is no clinically noninvasive method for high sensitivity, specificity and low-cost detection. A new microfluidic chip is designed for the detection of bladder cancer in a urine sample in the current research. Polydimethylsiloxane (PDMS) is used to form a channel using a cast molding method, with a sliding glass coverslip as the substrate. When the inner surface of the chip is modified by silanization to fix a specific antibody, urine samples and standard protein solutions are pumped into the chip to detect bladder cancer cells and biomarkers. The results show that the chip can quantitatively detect the difference in bladder cancer cells and biomarkers between patients' and volunteers' urine samples. The sensitivity and the specificity of the microfluidic chip are 87.1% and 100%, respectively. This study successfully establishes a novel microfluidic chip for the detection and diagnosis of bladder cancer, providing new ideas and a powerful platform for the detection and diagnosis of bladder cancer.