Outlook on optimizing ultramicroscopy imaging technique through optical characterization. Issue 9 (20th December 2016)

• Record Type: Journal Article
• Authors: Saghafi, Saiedeh; Becker, Klaus; Hahn, Christian; Pende, Marko; Jährling, Nina; Wanis, Martina; Sabdyusheva‐Litschauer, Inna; Foroughipour, Massih; Niendorf, Axel; Dodt, Hans‐Ulrich
• Is Part Of: Microscopy research and technique. Volume 81:Issue 9(2018)
• Page Start: 929
• Page End: 935
• Publication Date: 2016-12-20
• Subjects: light sheet microscopy -- optical parameters characterization -- Gaussian beam -- uniform beam profile; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22815
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Here, we present an optically optimized system for static ultramicroscopy imaging technique. The unit for generating an ultra‐thin light sheet employs aspheric and meso‐optical elements (meso‐aspheric system). An analytical as well as an experimental comparison between the light sheet produced by the standard system (using a rectangular slit aperture and one cylindrical lens) and the one produced by our latest optimized system, which converts a symmetrical Gaussian beam into an ultra‐thin light sheet is presented. Using the new light sheet in combination with our objective equipped with a modulator unit to compensate the refractive index mismatch between air and mediums with indices of 1.45–1.56, we present high resolution images of various biological samples that were chemically cleared using different methods. They demonstrate a marked improvement in quality, contrast and resolution. Abstract :