A PEGylated alternating copolymer with oxidation-sensitive phenylboronic ester pendants for anticancer drug delivery. (18th July 2019)

• Record Type: Journal Article
• Authors: Zhang, Yu; He, Pan; Liu, Xinming; Yang, Huailin; Zhang, Hongyu; Xiao, Chunsheng; Chen, Xuesi
• Is Part Of: Biomaterials science. Volume 7:Number 9(2019)
• Page Start: 3898
• Page End: 3905
• Publication Date: 2019-07-18
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c9bm00884e
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : An oxidation sensitive PEGylated alternating copolymer was designed for doxorubicin delivery with improved anticancer efficacy and low toxicity in vivo . Abstract : To target a response to a high oxidative stress environment of inflammatory or tumor sites, various reactive oxygen species (ROS) sensitive polymers have been developed as drug delivery systems. In this study, a novel oxidation sensitive copolymer, phenylboronic acid pinacol ester-functionalized methoxyl poly(ethylene glycol)- block -poly(phthalic anhydride- alter -glycidyl propargyl ether) (mPEG- b -P(PA- alt -GPBAe)), was designed and synthesized by ring-opening alternating copolymerization (ROAP) and click reaction. The copolymers could self-assemble into micelles in aqueous solution with an average size of 20.3 ± 9.3 nm, and are able to load hydrophobic anticancer drug (doxorubicin, DOX) with a high encapsulation efficiency of 75.2%. Interestingly, the encapsulated drug showed accelerated release in the trigger of H2 O2, or at low pH values. The copolymers have low cytotoxicity indicated by the 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay towards 4T1 cells, which showed cell viabilities of more than 80% with treatment of our copolymers at concentrations up to 0.5 mg mL −1 . The effective uptake of the drug-loaded micelles by 4T1 cells was investigated by confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) analysis. Finally, compared with free DOX, the DOX-loaded nanoparticles exhibited a better antitumor effect and had lower systemic toxicity in 4T1 tumor-bearing mice. Therefore, this new kind of copolymer acting as a stimuli-responsive nanocarrier should represent a promising therapeutic platform for cancer therapy.