Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids. (August 2019)

• Record Type: Journal Article
• Authors: Restelli, Laura; Marques, Andreia T.; Savoini, Giovanni; Invernizzi, Guido; Carisetti, Michela; Lecchi, Cristina; Bendixen, Emoke; Ceciliani, Fabrizio
• Is Part Of: Research in veterinary science. Volume 125(2019)
• Page Start: 451
• Page End: 458
• Publication Date: 2019-08
• Subjects: Adipose tissue -- Proteomics -- Fish oil -- Goat -- Stearic acid -- Peripartum; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2017.10.009
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative iTRAQ 2D-LC-MS/MS analysis. Twenty proteins were found to be differentially expressed in suckling kids' omental adipose tissue. Stearic acid induces changes in a higher number of proteins when compared to fish oil. Eleven proteins, namely AARS, ECl1, PMSC2, CP, HSPA8, GPD1, RPL7, OGDH, RPL24, FGA and RPL5 were decreased in ST-kids only. Four proteins, namely DLST, EEF1G, BCAP31 and RALA were decreased in FO-kids only, and one, NUCKS1, was increased. Four proteins, namely PMSC1, PPIB, TUB5 × 2 and EIF5A1, were be less abundant in both ST- and FO- kids. Most of the protein whose abundance was decreased in ST kids (10 out of 15) are involved in protein metabolism and catabolism pathways. Qualitative gene expression analysis confirmed that all the proteins identified by mass spectrometry, with the exception of FGA, were produced by adipose tissue. Quantitative gene expression analysis demonstrated that two proteins, namely CP, a minor acute phase protein, and ECl1, involved in fatty acid beta oxidation, were downregulated at mRNA level as well. ECl1 gene expression was downregulated in ST-kids AT as compared to Ctrl-kids and CP was downregulated in both ST- and FO-kids. The present results demonstrate that it is possible to influence adipose goat-kid proteome by modifying the maternal diet. Highlights: Quantitative proteomics was carried out on goat-kid omental adipose tissue after supplementing lactating mothers with different fatty acids. Different diets modified the omental adipose tissue proteome. Twenty proteins were found to be differentially expressed, of which only NUCKS1, was overexpressed. The downregulation of ECl1 and Ceruloplasmin was also confirmed at gene expression level. The results demonstrated that supplementing other diet with different PUFA may influence omental adipose tissue proteome.