A molecular-based method to estimate the risk associated with cyanotoxins and odor compounds in drinking water sources. (1st November 2019)

• Record Type: Journal Article
• Authors: Lu, Keng-Yu; Chiu, Yi-Ting; Burch, Michael; Senoro, Delia; Lin, Tsair-Fuh
• Is Part Of: Water research. Volume 164(2019)
• Publication Date: 2019-11-01
• Subjects: Response level -- Cyanotoxins -- Drinking water -- 2-Methylisoborneol -- Real-time PCR -- Risk; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2019.114938
• Languages: English
• ISSNs: 0043-1354

Abstract

Abstract: A biomolecular-based monitoring approach for the assessment of water quality hazards and risks associated with cyanobacteria was developed and validated in drinking reservoirs in Taiwan and the Philippines. The approach was based upon the measurement of gene abundances of toxigenic Microcystis and Cylindrospermopsis ; for cyanotoxins; and for aesthetically offensive earthy-musty odor compounds. This was compared to conventional monitoring approaches, which included cell enumeration by microscopy, and toxin and odor compound analysis by instrumental analytical methods and immunoassays as appropriate for the metabolites. The validation involved samples from ten major reservoirs on Taiwan's main island, nineteen reservoirs on the offshore islands, and Laguna de Bay in the Philippines. The gene-based approach was successfully validated statistically and compared to conventional widely utilized risk assessment schemes which have employed 'Alert Levels' for toxic cyanobacteria. In this case a new integrated scheme of 'Response Levels' is proposed which incorporates odor metabolite hazards in addition to cyanotoxins and is based upon gene copy numbers to derive quantitative triggers. The comprehensive scheme evaluated from these locations is considered to be more precise and efficient for both monitoring and as a risk assessment diagnostic tool, given that it offers the capacity for analysis of the abundance of genes for cyanobacterial metabolites in large numbers of natural water samples in a significantly reduced period of time compared to the approaches of cell enumeration by microscopy or metabolite analytical techniques. This approach is the first time both the hazard and risk for both odors and cyanotoxins from cyanobacteria have been considered together in a monitoring scheme and offers an improved means for determining the Response Levels in the risk assessment process for cyanobacteria and their metabolites in drinking water sources. Graphical abstract: Image 1 Highlights: A new approach was developed to quantify the risk for cyanotoxins and MIB in water. This gene-based method was tested in 30 lakes/reservoirs in Taiwan and Philippines. Both T&O and toxins from cyanobacteria are considered in a monitoring scheme. Compared to conventional methods, faster and more precise assessment can be obtained. The approach offers an improved means to quantify the risk levels for cyanobacteria.