The CaMKII inhibitor KN93-calmodulin interaction and implications for calmodulin tuning of NaV1.5 and RyR2 function. (September 2019)

• Record Type: Journal Article
• Authors: Johnson, Christopher N.; Pattanayek, Rekha; Potet, Franck; Rebbeck, Robyn T.; Blackwell, Daniel J.; Nikolaienko, Roman; Sequeira, Vasco; Le Meur, Remy; Radwański, Przemysław B.; Davis, Jonathan P.; Zima, Aleksey V.; Cornea, Razvan L.; Damo, Steven M.; Györke, Sandor; George, Alfred L.; Knollmann, Björn C.
• Is Part Of: Cell calcium. Volume 82(2019)
• Publication Date: 2019-09
• Subjects: AIP autocamtide-2-related inhibitor peptide -- CaM calmodulin -- CaMKII CaM dependent protein kinase type II -- DMSO dimethylsulfoxide -- HSQC heteronuclear single quantum coherence -- NaV1.5 voltage gated sodium channel 1.5 -- RyR2 ryanodine receptor calcium release channel isoform 2; Calcium calmodulin dependent kinase type II (CaMKII) -- CaMKII inhibition -- Calcium signaling -- KN93 -- KN92 -- autocamtide-2-inhibitor peptide; Calcium -- Metabolism -- Periodicals; Vertebrates -- Physiology -- Periodicals; Calcium -- Physiological effect -- Periodicals; Cell physiology -- Periodicals; Calcium in the body -- Periodicals; 572.516
• DOI: 10.1016/j.ceca.2019.102063
• Languages: English
• ISSNs: 0143-4160

Abstract

Graphical abstract: Highlights: Ca2+ /Calmodulin (CaM) binds the CaM dependent kinase II inhibitor (CaMKII) KN93. KN93 modifies NaV1.5 and RyR2 function independent of CaMKII. KN93 disrupts a high affinity interaction between NaV1.5 and CaM. When evaluating KN93 data, targets other than CaMKII need to be considered. Abstract: Here we report the structure of the widely utilized calmodulin (CaM)-dependent protein kinase II (CaMKII) inhibitor KN93 bound to the Ca 2+ -sensing protein CaM. KN93 is widely believed to inhibit CaMKII by binding to the kinase. The CaM-KN93 interaction is significant as it can interfere with the interaction between CaM and it's physiological targets, thereby raising the possibility of ascribing modified protein function to CaMKII phosphorylation while concealing a CaM–protein interaction. NMR spectroscopy, stopped-flow kinetic measurements, and x-ray crystallography were used to characterize the structure and biophysical properties of the CaM-KN93 interaction. We then investigated the functional properties of the cardiac Na + channel (NaV 1.5) and ryanodine receptor (RyR2). We find that KN93 disrupts a high affinity CaM-NaV 1.5 interaction and alters channel function independent of CaMKII. Moreover, KN93 increases RyR2 Ca 2+ release in cardiomyocytes independent of CaMKII. Therefore, when interpreting KN93 data, targets other than CaMKII need to be considered.