Neuroprotective effects of Brazilian green propolis on oxytosis/ferroptosis in mouse hippocampal HT22 cells. (October 2019)

• Record Type: Journal Article
• Authors: Takashima, Madoka; Ichihara, Kenji; Hirata, Yoko
• Is Part Of: Food and chemical toxicology. Volume 132(2019)
• Publication Date: 2019-10
• Subjects: Brazilian green propolis -- Artepillin C -- Kaempferide -- Antioxidant response element -- Oxytosis/Ferroptosis -- Neuroprotective effect; : ARE antioxidant response element -- DMEM Dulbecco's modified Eagle's medium -- DMSO dimethyl sulfoxide -- EEP ethanol extracts of Brazilian green propolis -- GAPDH glyceraldehyde 3 phosphate dehydrogenase -- GSH reduced glutathione -- HO-1 heme oxygenase-1 -- LDH lactate dehydrogenase -- Nrf2 the nuclear factor erythroid 2-related factor 2 -- ROS reactive oxygen species -- SOD superoxide dismutase -- WEP water extracts of Brazilian green propolis; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2019.110669
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Propolis is a sticky dark-colored substance produced by honey bees and comprises resin, balsam, wax, essential and aromatic oils, pollen, and several other substances; it is used in food and beverages to improve health and prevent diseases. We studied the neuroprotective effects of extracts of Brazilian green propolis in the mouse hippocampal cell line HT22. Ethanol extracts of Brazilian green propolis had a more potent preventive effect on oxidative stress-induced cell death, oxytosis/ferroptosis, in HT22 cells than water extracts of Brazilian green propolis, whereas it did not protect against anticancer drug-induced apoptotic cell death. Among the primary constituents of ethanol extracts of Brazilian green propolis, only artepillin C, kaempferide, and kaempferol demonstrated neuroprotective effects against oxytosis/ferroptosis. The flavonoid derivatives kaempferide and kaempferol are antioxidants with radical-scavenging abilities that additionally induce antioxidant response element-mediated transcriptional activity, suggesting that upregulation of endogenous antioxidant defense protects against oxidative stress. In contrast, artepillin C attenuated reactive oxygen species production; however, it did not induce antioxidant response element activation. These findings indicate that the ethanol extracts of Brazilian green propolis help to prevent oxidative stress-related neuronal cell death that is involved in the pathogenesis of several neurodegenerative diseases. Highlights: Extracts of Brazilian green propolis prevented oxytosis/ferroptosis in mouse hippocampal HT22 cells. We identified artepillin C, kaempferide, and kaempferol as the beneficial ingredients of Brazilian green propolis. Artepillin C, kaempferide, and kaempferol attenuate oxytosis/ferroptosis by multiple antioxidant mechanisms.