Analysis of global and gene-specific acetylation of histones in the liver of American bullfrog (Rana catesbeiana) tadpoles acclimated to low temperature. (August 2019)

• Record Type: Journal Article
• Authors: Ishihara, Akinori; Yamauchi, Kiyoshi
• Is Part Of: Journal of thermal biology. Volume 84(2019)
• Page Start: 488
• Page End: 495
• Publication Date: 2019-08
• Subjects: Acclimation -- Acclimatization -- Acetylation -- Gene expression -- Histone -- Temperature; acetyl-CoA acetyl coenzyme A -- ChIP chromatin immunoprecipitation -- CoA-SH free coenzyme A -- cyp7a1 cytochrome P450 7a1 -- H3K9me3 trimethylated histone H3 at lysine 9 -- H3K36me2 dimethylated histone H3 at lysine 36 -- H3K36me3 trimethylated histone H3 at lysine 36 -- H4ac acetylated histone H4 -- H3K9ac acetylated histone H3 at lysine 9 -- HAT histone acetyltransferase -- HDAC histone deacetylase -- HPLC high-performance liquid chromatography -- PAGE polyacrylamide gel electrophoresis -- RT-qPCR reverse transcription-quantitative polymerase chain reaction -- scd stearoyl-CoA desaturase -- SDS sodium dodecyl sulfate -- SEM standard error of the mean -- TBS Tris-buffered saline -- TH thyroid hormone; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2019.08.002
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: Severe environmental stressors such as low temperatures can affect gene expression by changing epigenetic states. American bullfrog ( Rana catesbeiana ) can overwinter as tadpoles, which can be active even in winter. However, the molecular mechanisms of epigenetic controls by which the tadpoles acclimate to low temperature are still unclear. In this study, we aimed to clarify the molecular mechanisms of global and gene-specific epigenetic regulations of low-temperature acclimation. We found that the global acetylation was decreased in the liver of bullfrog tadpoles acclimated to low temperature. The amounts of transcripts for two histone acetyltransferases were higher in the liver of tadpoles acclimated to low temperature than in those acclimated to warm temperature, while we observed no significant differences in the amounts of transcripts for histone deacetylases. We also found that the amounts of transcripts and acetylated histones on the specific temperature-responsive genes scd and cyp7a1 whose transcripts were increased and decreased, respectively, in response to low temperature were positively correlated. Cellular acetyl-CoA levels were higher in the liver of tadpoles acclimated to low temperature than in those acclimated to warm temperature. These results contradicted the states of histone acetylation, suggesting that bullfrog tadpoles have different epigenetic mechanisms to modify the histones when compared with those of other organisms such as reptiles and mammals, even though the relationship between the transcript amount and the states of histone acetylation on temperature-responsive genes was similar to that of mammals. Graphical abstract: Image 1 Highlights: Global acetylation was reduced in the liver of cold-acclimated tadpoles. Amounts of transcripts and acetylated histone on specific gene were correlated. Amounts of acetyl-CoA were higher in the liver of cold-acclimated tadpoles. Expression of genes tested might be regulated by other than that of HATs or HDACs.