Methyl protodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction. (October 2019)

• Record Type: Journal Article
• Authors: Ma, Yi-Long; Zhang, Ying-Shuo; Zhang, Fan; Zhang, Yuan-Yuan; Thakur, Kiran; Zhang, Jian-Guo; Wei, Zhao-Jun
• Is Part Of: Food and chemical toxicology. Volume 132(2019)
• Publication Date: 2019-10
• Subjects: Polygonatum sibiricum -- Methyl protodioscin -- Cell cycle -- Apoptosis -- Molecular mechanism; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2019.110655
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Methyl protodioscin (MPD) is a steroid saponin which has been well known for its pharmacological properties. Herein, we evaluated the anti-cancer activity of MPD for proliferation inhibition and apoptosis induction in Hela cells. MPD was purified from the rhizoma of Polygonatum sibiricum primarily and identified by HPLC, UPLC-TOF-MS/MS and NMR analysis, respectively. Results showed that MPD repressed cell proliferation at IC50 of 18.49 μM, altered cell morphology, arrested the cell cycle in G2/M phase, facilitated the generation of intracellular ROS and led to cell apoptosis in a concentration-dependent manner. Furthermore, MPD treatment promoted death receptor pathway and mitochondrial pathway efficiently. The inhibition of Caspase-8 and Caspase-9 proteins in these pathways abolished the apoptosis significantly, further demonstrated the mechanism of MPD-induced apoptosis. These findings offer novel information that MPD may be considered as a possible natural anti-cancerous agent in the form of functional foods or medicinal products. Graphical abstract: Image 1 Highlights: MPD arrested cell cycle in G2/M, affected the expression of cell cycle related genes in Hela cells. MPD treatment resulted in the generation of intracellular ROS of Hela cells. MPD regulate apoptosis of Hela cells via death receptor and mitochondrial pathway.