Effects of Amanita muscaria extract on different in vitro neurotoxicity models at sub-cellular and cellular levels. (October 2019)

• Record Type: Journal Article
• Authors: Kondeva-Burdina, Magdalena; Voynova, Maria; Shkondrov, Aleksandar; Aluani, Denitsa; Tzankova, Virginia; Krasteva, Ilina
• Is Part Of: Food and chemical toxicology. Volume 132(2019)
• Publication Date: 2019-10
• Subjects: Amanita muscaria -- Isolated rat brain microsomes -- Synaptosomes -- Mitochondria -- Neuroblastoma cell line SH-SY5Y -- Neuroprotection -- Human recombinant monoaminoxidase-B enzyme; AA ascorbic acid -- AME Amanita muscaria extract -- GSH reduced glutathione -- LPO lipid peroxidation -- MDA malonedialdehyde -- t-BuOOH tert-butyl hydro-peroxide -- PD Parkinson's disease -- SN substantia nigra -- 6-OHDA 6-hydroxydopamine -- LDH lactate dehydrogenase -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- PBS phosphate buffer solution -- TCA trichloroacetic acid -- TBA thiobarbituric acid -- DTNB 5, 5′-dithiobis-(2-nitrobenzoic acid) -- ROS reactive oxygen species -- DPPH 2, 2-diphenyl-1-picrylhydrazyl; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2019.110687
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Muscimol is the main compound found in Amanita muscaria . Several studies have proven that muscimol has suppressive effects on essential tremor, without impairing speech and coordination. The effects of muscimol in Parkinson-affected patients is also described in a number of studies. These studies describe the free radical scavenging and antioxidant activity of the mushroom extract. We have evaluated the possible neuroprotective effects of a standardized extract from A. muscaria, containing high amounts of muscimol, on different models of neurotoxicity in rat brain microsomes, mitochondria, synaptosomes as well as on neuroblastoma cell line SH-SY5Y. The possible inhibitory effect on human recombinant monoaminoxidase-B (hMAOB) enzyme was also studied. The extract revealed statistically significant neuroprotective effects on the in vitro neurotoxicity models and no inhibitory activity on hMAOB. Highlights: A. muscaria extract was evaluated at sub- and cellular level for neuroprotection. The extract did not show any hMAOB inhibitory activity. The in vitro study confirmed the antioxidant and neuroprotective effects of the extract.