Imaging of dense cell cultures by multiwavelength lens‐free video microscopy. Issue 5 (27th February 2017)

• Record Type: Journal Article
• Authors: Allier, C.; Morel, S.; Vincent, R.; Ghenim, L.; Navarro, F.; Menneteau, M.; Bordy, T.; Hervé, L.; Cioni, O.; Gidrol, X.; Usson, Y.; Dinten, J.‐M.
• Other Names: Holden Elena guestEditor.; Popescu Gabriel guestEditor.
• Is Part Of: Cytometry. Volume 91:Issue 5(2017)
• Page Start: 433
• Page End: 442
• Publication Date: 2017-02-27
• Subjects: lens‐free microscopy -- video cytometry -- dense cell cultures -- quantitative microscopy; Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.23079
• Languages: English
• ISSNs: 1552-4922

Abstract

Abstract: They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm 2 ) over a large field of view of 29.4 mm 2 . Combined with the compactness of the system which fits altogether inside an incubator, lens‐free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens‐free video‐microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry