A Case Study on the Keap1 Interaction with Peptide Sequence Epitopes Selected by the Peptidomic mRNA Display. (6th June 2019)

• Record Type: Journal Article
• Authors: Hirose, Hisaaki; Hideshima, Tomoki; Katoh, Takayuki; Suga, Hiroaki
• Is Part Of: Chembiochem. Volume 20:Number 16(2019)
• Page Start: 2089
• Page End: 2100
• Publication Date: 2019-06-06
• Subjects: high-throughput screening -- mRNA display -- peptides -- protein–protein interactions -- solid-phase synthesis; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201900039
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Many protein–protein and peptide–protein interactions (PPIs) play key roles in the regulation of biological functions, and therefore, the modulation of PPIs has become an attractive target of new drug development. Although a number of PPIs have already been identified, over 100 000 unknown PPIs are predicted to exist. To uncover such unknown PPIs, it is important to devise a conceptually distinct method from that of currently available methods. Herein, an mRNA display by using a total RNA library derived from various human tissues, which serves as a unique method to physically isolate peptide epitopes that potentially bind to a target protein of interest, is reported. In this study, selection was performed against Kelch‐like ECH‐associated protein (Keap1) as a model target protein, leading to a peptide epitope originating from astrotactin‐1 (ASTN1). It turned out that this ASTN1 peptide was able to interact with Keap1 more strongly than that with a known peptide derived from Nrf2; a well‐known, naturally occurring Keap1 binder. This case study demonstrates the applicability of peptidomic mRNA display for the rapid exploration of consensus binding peptide motifs and the potential for the discovery of unknown PPIs with other proteins of interest. Abstract : Library under construction : A peptidomic mRNA display method is reported, in which human endogenous peptide sequences can be screened against a protein of interest. Accordingly, a peptide sequence derived from astrotactin‐1 is identified as a Keap1‐binding peptide. The approach allows rapid high throughput and can be applied to various protein targets of interest.