Fluorometric 'switch-on' detection of heparin based on a system composed of rhodamine-labeled chitosan oligosaccharide lactate, and graphene oxide. (29th May 2018)

• Record Type: Journal Article
• Authors: Zhong, Linlin; Yun, Kyusik
• Is Part Of: Methods and applications in fluorescence. Volume 6:Number 3(2018:Sep.)
• Publication Date: 2018-05-29
• Subjects: Rhodamine B -- RhB-COL/GO -- heparin -- fluorescence quenching -- switch on; Fluorescence spectroscopy -- Periodicals; Fluorescence -- Periodicals; Spectrometry, Fluorescence -- Periodicals; Fluorescence -- Periodicals; Fluorescence; Fluorescence spectroscopy; Periodicals; 535.352
• DOI: 10.1088/2050-6120/aac51c
• Languages: English
• ISSNs: 2050-6120

Abstract

Abstract: A novel fluorescence 'Switch on' for the detection of heparin based on the RhB-COL/GO system was achieved. A strong fluorescence dye, Rhodamine B, was modified by chitosan oligosaccharide lactate (COL), which plays a major role in the formation of a positively charged RhB-COL complex. RhB-COL was soluble and stable in solution, which was characterized by using Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy. GO sheets quenched the fluorescence intensity of RhB-COL due to electron transfer from RhB to the GO surface. The decrease in fluorescence intensity of RhB-COL with increasing GO concentration was recorded using a Cary Eclipse fluorescence spectrophotometer. On the other hand, the addition of heparin replaced GO to bind with the RhB-COL surface via an electrostatic and noncovalent bond due to the abundant negative charge, which resulted in recovery of the fluorescence intensity. This RhB-COL/GO system possessed high selectivity and good sensitivity for the detection of heparin compared to other biomolecules, such as glycine, D-glucose, hyaluronic acid, L-glutamic acid, and ascorbic acid. The linear response toward heparin was measured over the range, 0–1.8 U · ml −1, with a low detection limit of 0.04 U · ml −1 . The satisfactory sensing performance of RhB-COL/GO for heparin supports new 'switch-on' sensor applications in heparin-related biomedical detection.