Selenium-containing polysaccharides from Lentinula edodes—Biological activity. (1st November 2019)

• Record Type: Journal Article
• Authors: Kaleta, Beata; Górski, Andrzej; Zagożdżon, Radosław; Cieślak, Marcin; Kaźmierczak-Barańska, Julia; Nawrot, Barbara; Klimaszewska, Marzenna; Malinowska, Eliza; Górska, Sandra; Turło, Jadwiga
• Is Part Of: Carbohydrate polymers. Volume 223(2019)
• Publication Date: 2019-11-01
• Subjects: ATCC American Type Culture Collection -- BHT butylated hydroxytoluene -- BRM biological response modifiers -- BSA bovine serum albumin -- DETBA 1, 3-diethyl-2-thiobarbituric acid -- ECGF endothelial cell growth factor -- FBS fetal bovine serum -- HUVECs human umbilical vein endothelial cells -- mAb monoclonal antibody -- NK natural killer cells -- PBMCs peripheral blood mononuclear cells -- PBS phosphate-buffered saline -- PHA phytohaemagglutinin -- PMA phorbol 12-myristate 13-acetate -- SAC Staphylococcus aureus Cowan -- SOD superoxide dismutase; Lentinula edodes -- Selenium -- Se-polysaccharides -- Immunosuppressants -- Lymphocytes T; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115078
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A selenium-containing polysaccharide, the analog of immunostimulatory drug lentinan was obtained. We expected enhanced immunostimulating activity by the selenated fraction versus lentinan. The Se-polysaccharide proved to be selective immunosuppressant with a strong antioxidant activity. The polysaccharide was non-toxic and strongly enhanced the cell viability. The biological effect depended on the Se incorporation and dissimilar structure versus lentinan. Abstract: We hypothesized that selenium(Se)-enriched polysaccharides would possess superior biological activity when compared to those non-enriched. To verify this hypothesis, we obtained by biotechnological methods a Se-enriched analog of Japanese anticancer drug lentinan and, as a reference, the non-Se-enriched fraction. We tested the effects of the obtained fractions on the proliferation of human peripheral blood mononuclear cells. The results suggested a selective immunosuppressive activity, non-typical for mushroom derived polysaccharides. Both fractions caused significant inhibition of human T lymphocyte proliferation induced by mitogens, without significant effects on B lymphocytes. The inhibitory effect was not due to the toxicity of the examined polysaccharides. In normal (HUVEC) or malignant (HeLa) cells tested fractions significantly enhanced cell viability and protected the cells from oxidative stress conditions. However, we observed no effect of the polysaccharide fractions on the production of reactive oxygen species by granulocytes in vitro. The selenium content increased the biological activity of the tested polysaccharide fractions.