Self-assembly of bacteria cellulose hydrogels carrying multiple carbohydrate appendages to visualize carbohydrate-carbohydrate interactions. (1st November 2019)

• Record Type: Journal Article
• Authors: Yoshida, Keisuke; Kaino, Mizuki; Sekiguchi, Maki; Chigira, Naoto; Amano, Yoshitsugu; Inokuchi, Mayu; Li, Qintong; Hasegawa, Teruaki
• Is Part Of: Carbohydrate polymers. Volume 223(2019)
• Publication Date: 2019-11-01
• Subjects: GSLs glycosphingolipids -- CCIs carbohydrate–carbohydrate interactions -- BC bacterial cellulose -- GNPs gold nanoparticles -- SNPs silica nanoparticles -- TEM transmission electron microscopy -- DIPEA NN-diisopropylethylamine -- DMC NN-dimethyl-2-chloroimidazolium chloride -- CDMBI 2–chloro–13–dimethyl–1H–benzimidazol–3–ium chloride -- RCA Ricinus Communis Agglutinin -- MAL–II Maackia amurensis Lectin II -- PBS phosphate buffed salts; Carbohydrate–carbohydrate interaction -- Hydrogel -- Bacterial cellulose -- Huisgen cycloaddition; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115062
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Bacteria cellulose hydrogels carrying lactosides or 3'-sialyllactosides. Carbohydrate-carbohydrate interactions that were monitored with naked eye. Ion-selectivity (Ca 2+, Mg 2+, or Na + ) to induce carbohydrate-carbohydrate interactions. Abstract: Nata de coco was chemically modified to afford the bacterial cellulose hydrogels carrying terminal alkynes. The resultant hydrogels were then converted into hydrogels carrying lactosides or those carrying α–2, 3–sialyllactosides by the Cu + –catalyzed alkyne–azide cyclization. The stable homo association of the hydrogels carrying lactosides was observed in an aqueous solution containing Ca 2+, thereby demonstrating the Ca 2+ –mediated lactoside–lactoside interactions. Ca 2+ also stabilized the hetero associations among the hydrogels carrying lactosides and those carrying α–2, 3–sialyllactosides, thereby also demonstrating the Ca 2+ -induced interactions between the lactosides and the α–2, 3–sialyllactosides. The sizes of these hydrogels were of the order of ca. 5 mm, and their associations could thus be readily monitored with the naked eye.