Structural characterization and in vitro hepatoprotective activity of polysaccharide from pine nut (Pinus koraiensis Sieb. et Zucc.). (1st November 2019)

• Record Type: Journal Article
• Authors: Qu, Hang; Gao, Xin; Zhao, Hai-Tian; Wang, Zhen-Yu; Yi, Juan-Juan
• Is Part Of: Carbohydrate polymers. Volume 223(2019)
• Publication Date: 2019-11-01
• Subjects: PNP40 pine nut (Pinus koraiensis Sieb. et Zucc. polysaccharide PNP precipitated with 40% ethanol -- CCl4 carbon tetrachloride -- OS oxidative stress -- Ara arabinose -- Gal galactose -- Glc glucose -- Rha rhamnose -- Man mannose -- Xyl xylose -- TFA trifluoroacetic acid -- SOD superoxide dismutase -- GSH-Px glutathione peroxidase -- GSH glutathione -- MDA malondialdehyde -- ALT alanine aminotransferase -- AST aspartate aminotransferase -- Mw molecular weights -- HPGPC high performance gel permeation chromatography -- UV ultraviolet -- FT-IR Fourier-transform infrared -- GC gas chromatography -- GC/MS gas chromatography-mass spectrometer -- NMR nuclear magnetic resonance -- COSY 1H/1H correlation spectroscopy -- HSQC 1H/13C heteronuclear single-quantum correlation spectroscopy -- HMBC heteronuclear multiple-bond coherence spectroscopy -- XRD X-ray Diffractometer -- SEM scanning electron microscope -- AFM atomic force microscopy; Arabinose (PubChem CID: 5460291) -- Galactose (PubChem CID: 6036) -- Glucose (PubChem CID: 5793) -- Mannose (PubChem CID: 18950) -- Rhamnose (PubChem CID: 25310) -- Xylose (PubChem CID: 135191) -- Trifluoroacetic acid (PubChem CID: 6422) -- Sodium borogydride (PubChem CID: 4311764); Pinus koraiensis Sieb. et Zucc. -- Pine nut polysaccharide -- Separation and purification -- Structural characterization -- Hepatoprotective effect -- L02 cells; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115056
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A new polysaccharide is isolated from Pinus koraiensis Sieb. et Zucc. pine nuts. The compositions and structural characterization of PNP40c-1 are identified. PNP40c-1 shows hepatoprotective effect against CCl4 -induced liver damage in vitro . Abstract: A new purified polysaccharide (PNP40c-1) with a molecular weight of 2.06 × 10 5 Da was obtained from pine nuts ( Pinus koraiensis Sieb. et Zucc. ). Structural analysis indicated that PNP40c-1 is a homogeneous heteropolysaccharide composed of arabinose, rhamnose and glucose in a molar ratio of 2.98:1.00:0.52. The major backbone consisted of →3, 4)- α -l -Ara p (1→, →4)- α -l -Ara p 3Me(1→, →3)- α -l -Rha p (1→ and →6)- β -d -Glc p (1→, and the side chain is β -d -Glc p -(1→ linked at C4-position of →3, 4)- α -l -Ara p (1→. In addition, the hepatoprotective effect of PNP40c-1 was investigated by human hepatocyte cell line L02 treated with carbon tetrachloride (CCl4 ). Results suggested that PNP40c-1 could protect L02 cells from CCl4 -induced damage by enhancing the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), increasing the level of non-enzymatic antioxidant glutathione (GSH), suppressing lipid perioxidation and further reducing the leakage of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Hence, PNP40c-1 could be a promising functional food to serve as hepatoprotective agent.