Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability. (1st November 2019)

• Record Type: Journal Article
• Authors: Li, Wei; Liu, Liming; Tian, Huafeng; Luo, Xiaogang; Liu, Shilin
• Is Part Of: Carbohydrate polymers. Volume 223(2019)
• Publication Date: 2019-11-01
• Subjects: WPI whey protein isolate -- ALG alginate -- FP freeze-drying protectant -- LP Lactobacillus plantarum -- CL cellulose microgel with LP -- AG Ca-alginate gel with LP -- ACG Ca-alginate/cellulose gel with LP -- ACFP Ca-alginate/cellulose/cryoprotectant gel with LP -- AFP Ca-alginate/cryoprotectant gel with LP -- CFP mixture of cellulose microgel and cryoprotectant with LP -- ACT Ca-alginate/cellulose/trehalose gel -- ACW Ca-alginate/cellulose/WPI gel -- ACTW Ca-alginate/cellulose trehalose/WPI gel; Cellulose -- Probiotics -- Controlled release -- Storage stability; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115065
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Porous cellulose microgels provided a house for L. plantarum . The ACFP capsules had a good acid tolerance and storage stability of 160 days. The encapsulated L. plantarum had a controlled release behavior. Abstract: Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function. Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process. The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region. The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 10 6 cfu/mL at 210 min. In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 10 6 cfu/mL after the storage of 160 days at 4 °C. The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.