High incidence of intact or fragmented immunoglobulin in urine of patients with multiple myeloma. Issue 12 (2nd December 2015)

• Record Type: Journal Article
• Authors: Kraj, Maria; Kruk, Barbara; Lech-Marańda, Ewa; Warzocha, Krzysztof; Prochorec-Sobieszek, Monika
• Is Part Of: Leukemia & lymphoma. Volume 56:Issue 12(2015:Dec.)
• Page Start: 3348
• Page End: 3356
• Publication Date: 2015-12-02
• Subjects: Free light chains -- intact immunoglobulin -- multiple myeloma -- urine immunoglobulin; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.3109/10428194.2015.1037753
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract : In this prospective study we determined the incidence of intact/fragmented immunoglobulin and Bence Jones protein in urine immunofixation using Sebia reagents and Hydrasys TM 2 apparatus and compared the results to concentrations of serum free light chains (FLC) assessed using Siemens BN TM II nephelometer and the immunoassay Freelite (Binding Site) in 289 patients with multiple myeloma at diagnosis. It was found that in one third of IgG, IgA and IgD myeloma patients, intact/fragmented immunoglobulin can be detected in urine and is connected with impaired renal function and reduced survival. Urine immunofixation detects monoclonal protein (FLC and intact/fragmented immunoglobulin) in 66–79% of IgG and IgA myeloma patients while serum FLC immunoassay detect it in 82–94% of IgG and IgA myeloma patients. However, the latter method is inadequate for detection of intact/fragmented immunoglobulin in urine. Serum FLC immunoassay and urine immunofixation are complementary methods in diagnosing and monitoring monoclonal protein in patients with myeloma.