Bioassay-guided isolation and identification of bioactive compound from aerial parts of Luffa acutangula against lung cancer cell line NCI-H460. (4th July 2015)

• Record Type: Journal Article
• Authors: Vanajothi, Ramar; Srinivasan, Pappu
• Is Part Of: Journal of receptor and signal transduction research. Volume 35:Number 4(2015)
• Page Start: 295
• Page End: 302
• Publication Date: 2015-07-04
• Subjects: Active fraction LA/FII -- apoptosis -- Luffa acutangula -- mitochondrial membrane potential -- NCI-H460 -- ROS; Cell receptors -- Periodicals; Cellular signal transduction -- Periodicals; 571.6
• DOI: 10.3109/10799893.2014.977451
• Languages: English
• ISSNs: 1079-9893

Abstract

Abstract: Luffa acutangula (Cucurbitaceae) is widely used as a traditional medicine in India and was reported to possess various pharmacological activities including its anti-proliferative effects. In this study, the bioactive compound of ethanolic extract of L. acutangula (LA) was isolated using bioassay-guided approach. Five major fractions were collected and evaluated for their anti-proliferative activity against non-small cell lung cancer cells (NCI-H460). Among the test fractions, the fraction LA/FII effectively decreased the growth of cancer cells with IC50 values of 10 µg/ml concentration. Furthermore, it significantly increased intracellular reactive oxygen species and decreased the mitochondrial membrane potential. The apoptogenic activity of fraction LA/FII was confirmed by cell shrinkage, membrane blebbing and formation of apoptotic bodies. A single bioactive compound was isolated from the active faction, LA/FII and subsequently identified as 1, 8 dihydroxy-4-methylanthracene 9, 10-dione (compound1 ) by comparing its spectral data [Ultraviolet (UV), Infrared (IR), Nuclear magnetic resonance (NMR) and Electrospray Ionization-Mass Spectroscopy (ESI-MS)] with literature values. This is the first report on the isolation of compound1 from this plant.