Transplant of ex vivo incubated bone marrow with rIL -7 for the enhancement of immuno-hematopoietic reconstitution. Issue 12 (2nd December 2015)

• Record Type: Journal Article
• Authors: Abdul-Hai, Ali; Yehuda, Aryeh Ben; Slavin, Shimon; Or, Reuven
• Is Part Of: Leukemia & lymphoma. Volume 56:Issue 12(2015:Dec.)
• Page Start: 3387
• Page End: 3392
• Publication Date: 2015-12-02
• Subjects: bone marrow transplant -- immune reconstitution -- interleukin-7 (IL-7) -- stem cell transplant; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.3109/10428194.2015.1016935
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract : Interleukin-7 (IL-7) is a critical cytokine in early B and T cell development. Peripheral T cell expansion and thymopoiesis and is a result of the ongoing reconstitution from uncommitted stem cells after transplant. We investigated the efficacy of ex vivo incubated bone marrow cells treated with recombinant human IL-7 (rIL-7) on subsequent in vivo murine models of syngeneic bone marrow (BM) transplant. After ex vivo culture with rIL-7, we observed a 1½-fold increase in BM cellularity; this increase was associated with an enhanced reconstitution of bone marrow cells and thymocytes at 45 days post-transplant. In addition to increased cellularity, lymphocytes from mice transplanted with cultured rIL-7showed enhanced proliferative responses to mitogenic stimulation. These findings suggest rIL-7 to be a promising agent for the clinical application of treating immune deficiency and enhancing immuno-hematopoietic reconstitution of the stem cell auto/allograft.