Anti-leukemic activities of alcoholic extracts of two traditional Indian medicinal plants. Issue 11 (2nd November 2015)

• Record Type: Journal Article
• Authors: Bhargava, Shipra; Malhotra, Hemant; Rathore, Om Singh; Malhotra, Bharti; Sharma, Pratibha; Batra, Amla; Sharma, Asha; Chiplunkar, Shubhada V.
• Is Part Of: Leukemia & lymphoma. Volume 56:Issue 11(2015:Nov.)
• Page Start: 3168
• Page End: 3182
• Publication Date: 2015-11-02
• Subjects: Chronic myeloid leukemia (CML) -- anticancer activity -- cell line -- SRB assay -- MTT assay; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.3109/10428194.2015.1026813
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract : The present work aimed to investigate the anticancer in vitro activity of two plants commonly used in traditional Indian medicine: Zingiber officinale Roscoe and Nerium oleander L. The extracts of these plants were tested in vitro on several human leukemic cell lines, K562, THP-1, MOLT-4 and Jurkat. Cell growth inhibition was observed for both plant extracts with 50% inhibitory concentration (IC50 ) values ranging between 1 and 28 μg/mL using SRB (sulphorodamine B) and MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assays. Enhanced cell growth inhibition was observed when the extracts were combined with imatinib. Exposed cells showed cell cycle arrest, DNA damage and cytochrome c release, indicating that the mechanism of cytotoxicity could be via mitochondrial mediated apoptotic pathways. Combination of the extracts of these plants with standard cancer treatment may be a way of enhancing responses. Clinical studies in patients with chronic myeloid leukemia are planned at our center.