Identification of haptoglobin switch-on status in archived placental specimens indicates antenatal exposure to inflammation and potential participation of the fetus in triggering preterm birth. (February 2018)

• Record Type: Journal Article
• Authors: McCarthy, Megan E.; Buhimschi, Catalin S.; Hardy, John T.; Dulay, Antonette T.; Laky, Christine A.; Bahtyiar, Mert-Ozan; Papanna, Ramesha; Zhao, Guomao; Buhimschi, Irina A.
• Is Part Of: Placenta. Volume 62(2018)
• Page Start: 50
• Page End: 57
• Publication Date: 2018-02
• Subjects: Fetus -- Infection -- Amniotic fluid -- Sepsis -- Prostaglandins -- Phenotype; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2017.12.017
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Objective: Haptoglobin (Hp) has key immunoregulatory roles that vary with phenotype (Hp1-1, Hp2-1, Hp2-2). Cord blood Hp expression is switched-off in the normal fetus. We hypothesized that in the setting of fetal inflammation placenta becomes inundated with Hp of fetal origin that in turn modulates the output of PGE2 and MMP-9 in a phenotype dependent manner. Methods: Placentas from 40 pregnancies complicated by preterm birth (PTB) (<37 weeks), without (n = 15) or with (n = 25) intra-amniotic infection and histological chorioamnionitis (HCA) were scored for intensity of Hp immunostaining. Hp mRNA levels were evaluated by PCR. Cord blood Hp levels, switch-on status and phenotypes were determined by ELISA and Western blotting. Using a villous trophoblast explant system we investigated if Hp can modulate the release of PGE2 and MMP-9 in the presence or absence of lipopolysaccharide (LPS). Results: All cases with HCA had positive Hp immunoreactivity within fetal vascular spaces. Hp staining intensity correlated with cord blood Hp levels and IL-6. Placentas with and without HCA had similar Hp mRNA levels suggesting Hp immunostaining in the fetal spaces is of fetal rather than placental origin. Both Hp1-1 and Hp2-2 up-regulated PGE2 release in the presence of LPS (2-fold over the LPS level, P < .05), without affecting MMP-9 concentrations. Conclusions: Fetal Hp switch-on status, a marker of antenatal exposure to intra-amniotic infection/inflammation, can be reliably established through evaluation of archived placental specimens. In the setting of infection/inflammation, Hp enhances placental PGE2 output thereby supporting the role of the fetus in triggering parturition. Highlights: Fetuses exposed to infection and inflammation in utero produce haptoglobin. The precocious onset of haptoglobin synthesis by the fetus may participate to labor. Haptoglobin switch status can be determined from archived placental specimens.