D-LL-31 in combination with ceftazidime synergistically enhances bactericidal activity and biofilm destruction in Burkholderia pseudomallei. Issue 5 (28th May 2019)

• Record Type: Journal Article
• Authors: Wongkaewkhiaw, Saharut; Taweechaisupapong, Suwimol; Anutrakunchai, Chitchanok; Nazmi, Kamran; Bolscher, Jan G.M.; Wongratanacheewin, Surasakdi; Kanthawong, Sakawrat
• Is Part Of: Biofouling. Volume 35:Issue 5(2019)
• Page Start: 573
• Page End: 584
• Publication Date: 2019-05-28
• Subjects: Antimicrobial activity -- cathelicidin peptides -- D-enantiomer peptide -- bacterial biofilm -- melioidosis; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2019.1632835
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: Melioidosis is a severe disease caused by Burkholderia pseudomallei . The biofilm of B. pseudomallei acquires resistance to several antibiotics and may be related to relapse in melioidosis patients. Here, the killing activity of antimicrobial peptides (LL-37, LL-31) and the D-enantiomers (D-LL-37, D-LL-31) in combination with ceftazidime (CAZ) against B. pseudomallei 1026b, H777 and a biofilm mutant M10, derived from H777 grown under biofilm-stimulating conditions was observed. Using static conditions, D-LL-31 exhibited the strongest killing activity against the three isolates in a dose-dependent manner. IC50 values for D-LL-31 ranged from 1 to 6 µM, for isolates M10, H777, and 1026b, respectively. Moreover, D-LL-31 combined with CAZ synergistically decreased the IC50 values of the peptide and antibiotic and caused also disruption of biofilms of B . pseudomallei 1026b under flow conditions. Thus a combination of D-LL-31 and CAZ may enhance the efficacy of the currently used antibiotic treatments against B. pseudomallei.