High-speed video capillaroscopy method for imaging and evaluation of moving red blood cells. (May 2018)

• Record Type: Journal Article
• Authors: Gurov, Igor; Volkov, Mikhail; Margaryants, Nikita; Pimenov, Aleksei; Potemkin, Andrey
• Is Part Of: Optics and lasers in engineering. Volume 104(2018)
• Page Start: 244
• Page End: 251
• Publication Date: 2018-05
• Subjects: Biomedical imaging -- Capillary -- Red blood cell velocity -- Video capillaroscopy; Lasers in engineering -- Periodicals; Optical measurements -- Periodicals; Optics -- Periodicals; Lasers en ingénierie -- Périodiques; Mesures optiques -- Périodiques; Optique -- Périodiques; 621.36605
• DOI: 10.1016/j.optlaseng.2017.09.003
• Languages: English
• ISSNs: 0143-8166

Abstract

Highlights: The proposed method allows to evaluate spatial capillary area and central line independently on properties of individual capillary. Simultaneous blood flow velocity records related to a few neighbor capillaries into a capillary net are demonstrated for the first time. Abstract: The video capillaroscopy system with high image recording rate to resolve moving red blood cells with velocity up to 5 mm/s into a capillary is considered. Proposed procedures of the recorded video sequence processing allow evaluating spatial capillary area, capillary diameter and central line with high accuracy and reliability independently on properties of individual capillary. Two-dimensional inter frame procedure is applied to find lateral shift of neighbor images in the blood flow area with moving red blood cells and to measure directly the blood flow velocity along a capillary central line. The developed method opens new opportunities for biomedical diagnostics, particularly, due to long-time continuous monitoring of red blood cells velocity into capillary. Spatio-temporal representation of capillary blood flow is considered. Experimental results of direct measurement of blood flow velocity into separate capillary as well as capillary net are presented and discussed.