Study on the mechanism underlying Al-induced hepatotoxicity based on the identification of the Al-binding proteins in liver. Issue 8 (25th July 2019)

• Record Type: Journal Article
• Authors: Ding, Yixin; Tang, Jinlei; You, Xun; Zhang, Xiongfeng; Wang, Guangliang; Yao, Congying; Lin, Mibin; Wang, Xuerui; Cheng, Dai
• Is Part Of: Metallomics. Volume 11:Issue 8(2019)
• Page Start: 1353
• Page End: 1362
• Publication Date: 2019-07-25
• Subjects: Metals -- Physiological effect -- Periodicals; 572.51
• DOI: 10.1039/c9mt00150f
• Languages: English
• ISSNs: 1756-5901

Abstract

Abstract : Aluminum (Al) is the most abundant metal element in the earth's crust, and is implicated in the pathogenesis of liver lesions. Abstract : Aluminum (Al) is the most abundant metal element in the earth's crust, and is implicated in the pathogenesis of liver lesions. However, the mechanisms underlying Al 3+ -induced hepatotoxicity are still largely elusive. Based on analysis with native gel electrophoresis, Al 3+ plus 8-hydroxyquinoline staining and LC-MS/MS, the proteins with high Al 3+ affinity were identified to be carbamoyl-phosphate synthase, adenosylhomocysteinase, heat shock protein 90-alpha, carbonic anhydrase 3, serum albumin and calreticulin. These proteins are involved in physiological processes such as the urea cycle, redox reactions, apoptosis and so on. Then we established an Al 3+ -treated rat model for biochemical tests, morphology observation and Ca 2+ homeostasis analysis, in order to evaluate the extent of oxidative damage, hepatic histopathology and specific indicators of Al 3+ -related proteins in liver. Our findings indicated the high-affinity interactions with Al 3+ perturbed the normal function of the above proteins, which could account for the mechanism underlying Al 3+ -induced hepatotoxicity.