DNA-templated copper nanoclusters obtained via TdT isothermal nucleic acid amplification for mercury(ii) assay. Issue 32 (30th July 2019)

• Record Type: Journal Article
• Authors: He, Jing-Lin; Wang, Xing-Xing; Mei, Ting-Ting; Wu, Ling; Zeng, Ju-Lan; Wang, Jian-Hui; Wang, Jianxiu; Yu, Donghong; Cao, Zhong
• Is Part Of: Analytical methods. Volume 11:Issue 32(2019)
• Page Start: 4165
• Page End: 4172
• Publication Date: 2019-07-30
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay01214a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : The Hg 2+ fluorescence sensor based on TdT isothermal amplification DNA templated CuNCs was successfully constructed. Abstract : Herein, DNA-templated copper nanoclusters (CuNCs) synthesized by terminal deoxynucleotidyl transferase (TdT) isothermal nucleic acid amplification (INAA) were proposed for Hg 2+ assay. TdT recognized the 3′-OH end of primers and subsequently prolonged the single strand poly-T at 3′-OH with the assistance of deoxythymidine triphosphate (dTTP). The generated products coordinated with the copper ions to form DNA-templated CuNCs, resulting in remarkable fluorescence. The introduction of Hg 2+ made the primers form a T–Hg 2+ –T mismatch duplex, which prevented the capture of the 3′-OH end by TdT. The poly-T single strand prolongation reaction was suspended; this led to fluorescence quenching. Hg 2+ could be detected at a concentration as low as 76 pM with a significant linear range from 0.2 to 500 nM. This method, performed in one tube in one step, does not involve washing and separation steps, which provides a novel insight into the ultra-sensitive detection of toxic analytes in water quality applications.