Changes in lipid classes of Drosophila melanogaster in response to selection for three stress traits. (August 2019)

• Record Type: Journal Article
• Authors: Ko, Li; Harshman, Lawrence; Hangartner, Sandra; Hoffmann, Ary; Kachman, Steve; Black, Paul
• Is Part Of: Journal of insect physiology. Volume 117(2019)
• Publication Date: 2019-08
• Subjects: BHT butylated hydroxytoluene -- DAGs diacylglycerols -- ESI MS electrospray mass spectrometry -- ESI MS/MS electrospray tandem mass spectrometry -- FFA free fatty acids -- HPLC high pressure liquid chromatography -- KLRC Kansas Lipidomics Research Center -- PA phosphatidic acid -- PC phosphatidylcholine -- PE phosphatidylethanolamine -- PG phosphatidylglycerol -- PI phosphatidylinositol -- PS phosphatidylserine -- TAGs triacylglycerols; Drosophila melanogaster -- Lipid profile -- Lipid metabolome -- Laboratory selection -- Stress selection -- Stress resistance -- Heat -- Desiccation -- Chill-coma; Insects -- Physiology -- Periodicals; Insectes -- Physiologie -- Périodiques; Insects -- Physiology; Periodicals; 571.157
• DOI: 10.1016/j.jinsphys.2019.103890
• Languages: English
• ISSNs: 0022-1910

Abstract

Graphical abstract: Highlights: Heat increased DAG amounts. Cold increased only PA amounts. Dry changed many lipids. Abstract: Laboratory selection on environmental stress traits is an evolutionary approach that is informative in the context of understanding stress adaptation. Here we characterize changes in a lipidome of Drosophila melanogaster in lines selected for increased heat (elevated heat knockdown refractoriness), cold (decreased time to recover from chill-coma) and desiccation survival. Selection for desiccation resistance resulted in changes in multiple lipid classes used to characterize a lipidome. This included a decrease in triacylglycerols (TAGs) which is relevant to interpretation of storage lipid levels in previous D. melanogaster desiccation survival selection experiments. Chill-coma recovery rate selection was expected to show extensive changes in lipid classes, but only phosphatidic acids exhibited significant change. Selection for increased heat knockdown resistance resulted in a substantial change in the abundance of a class of lipids (diacylglycerols) which could play a role in mediating the heat shock response or result in an increase in neutral lipid mobilization.