The effects of vitamins and selenium mixture against brain tissue induced by d‐galactosamine. Issue 8 (25th April 2019)

• Record Type: Journal Article
• Authors: Tunali, Sevim; Catal, Tunc; Bolkent, Sehnaz; Yanardag, Refiye
• Is Part Of: Journal of biochemical and molecular toxicology. Volume 33:Issue 8(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-04-25
• Subjects: antioxidant -- brain -- enzymes -- galactosamine -- oxidative stress; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Toxicology -- Periodicals; 574
• DOI: 10.1002/jbt.22347
• Languages: English
• ISSNs: 1095-6670

Abstract

Abstract: Brain damage is a major complication of fulminant hepatic failure.d ‐Galactosamine (d ‐GalN)‐induced liver toxicity causes damage to brain. The effects of vitamins and selenium mixture againstd ‐GalN stimulated brain injury were investigated in this study. Sprague‐Dawley female rats aged 2.0‐2.5 months were used for the study. The rats were divided into four categories. A 0.9% NaCl solution was intraperitoneally given to the experimental rats in the first group. Using gavage technique, the second group of animals were subjected to a formulation consisting of 100 mg·kg −1 ·day −1 vitamin C, 15 mg·kg −1 ·day −1 of β‐carotene, 100 mg·kg −1 ·day −1 of α‐tocopherol in addition to 0.2 mg·kg −1 ·day −1 of sodium selenate for 3 days. The third group was given a single dose ofd ‐GalN hydrochloride at the concentration of 500 mg·kg −1 through a saline injection. The final group was given similar concentrations of both the antioxidant combination andd ‐GalN. Tissue samples were collected under ether anesthesia. The rats treated withd ‐GalN showed brain damage; increased myeloperoxidase, catalase, glutathione peroxidase, glutathione‐S‐transferase, lactate dehydrogenase, and superoxide dismutase activities; and decreased glutathione levels. Treatment with vitamins and selenium combination resulted in alleviation of these alterations in the rats. These findings suggest that administration of the vitamins and selenium combination suppresses oxidative stress and protects brain cells from injury induced byd ‐GalN.