Intron length polymorphism of β‐tubulin genes of Aegilops biuncialis Vis. (17th November 2017)

• Record Type: Journal Article
• Authors: Rabokon, Anastasiia; Demkovych, Andrii; Sozinov, Alexei; Kozub, Natalia; Sozinov, Igor; Pirko, Yaroslav; Blume, Yaroslav
• Is Part Of: Cell biology international. Volume 43:Number 9(2019)
• Page Start: 1031
• Page End: 1039
• Publication Date: 2017-11-17
• Subjects: β‐tubulin -- Aegilops -- intron length polymorphism -- plants genotyping -- tubulin base polymorphism; Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10886
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: Intron‐specific DNA polymorphism is present among plant β‐tubulin gene family members and is considered to be one of the molecular markers based on the difference of tubulin introns length assayed both separately (TBP: 1st intron) or in combination (h‐TBP: 1st and 2nd introns). These two approaches are possibly useful for wheat breeding programs, since TBP and h‐TBP help to differentiate between the accessions of Aegilops biuncialis Vis., a wild relative of wheat. PCR‐derived polymorphic fragments were resolved by PAGE electrophoresis. The length of amplicons varied significantly (395–3900 bp for TBP and 466–3440 bp for h‐TBP), while the numbers of polymorphic bands were 21 for TBP and 23 for h‐TBP, respectively. PIC mean value was circa 0.3. Dendrograms constructed on the basis of the Nei and Li coefficient with the high bootstrap support reveal a similar order of hierarchy for the samples analyzed using both methods. Thus, both techniques uncover DNA polymorphism level sufficiently high to distinguish different accessions of Ae. biuncialis Vis.