A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa. Issue 8 (23rd June 2019)

• Record Type: Journal Article
• Authors: Belala, Réda; Briand‐Amirat, Lamia; Martinot, Aline; Thorin, Chantal; Michaud, Sandrine; Desherces, Serge; Youngs, Curtis R.; Bencharif, Djemil
• Is Part Of: Reproduction in domestic animals. Volume 54:Issue 8(2019)
• Page Start: 1131
• Page End: 1138
• Publication Date: 2019-06-23
• Subjects: canine spermatozoa -- cryopreservation -- egg yolk plasma -- in vitro fertility tests -- lyophilization; Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/rda.13476
• Languages: English
• ISSNs: 0936-6768

Abstract

Abstract: The current study aimed to explore the potential usefulness of liquid or lyophilized egg yolk plasma (EYP) as a substitute for low‐density lipoproteins (LDL) for cryopreservation of canine spermatozoa. In the first experiment, a total of 20 ejaculates harvested from six Beagles were frozen in extenders containing 6% LDL (control) or liquid or lyophilized EYP at one of three concentrations (20%, 40% or 60%). Motility parameters were assessed 10 min after thawing using computer‐assisted sperm analysis. For both liquid and lyophilized EYP, the 40% concentration yielded motility similar ( p > 0.05) to that observed with the control extender. In the second experiment, 12 ejaculates collected from the same six dogs were frozen in 6% LDL (Control), 40% liquid EYP or 40% lyophilized EYP extenders. Spermatozoal membrane integrity (hypo‐osmotic swelling test [HOSt] and SYBR14/propidium iodide [PI] staining), acrosome integrity (FITC‐ Pisum sativum agglutinin staining) and DNA integrity (acridine orange staining) characteristics were evaluated 10 min after thawing. Both liquid and lyophilized 40% EYP‐based extenders successfully preserved all assessed integrity parameters as efficiently as the control. Results of this study suggest that lyophilized EYP is a viable alternative to LDL in freezing extenders for dog semen.