The implication of single‐nucleotide polymorphisms in ovarian hyperstimulation syndrome. Issue 8 (22nd May 2019)

• Record Type: Journal Article
• Authors: Ghaderian, Sayyed Mohammad Hossein; Akbarzadeh, Reza; Mohajerani, Fatemeh; Khodaii, Zohreh; Salehpour, Saghar
• Is Part Of: Molecular reproduction and development. Volume 86:Issue 8(2019)
• Page Start: 964
• Page End: 971
• Publication Date: 2019-05-22
• Subjects: assisted reproductive technology -- genetic variations -- infertility -- ovarian hyperstimulation syndrome; Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23171
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract: Ovarian hyperstimulation syndrome (OHSS) is an undesirable complication in the course of ovarian stimulation. This kind of stimulation is aimed at acquiring a sufficient number of high‐quality oocytes in in vitro fertilization (IVF). Whereas the predisposition to OHSS could be impacted by genetic polymorphisms in susceptible genes, the present study has been jointly conducted with an Iranian cohort to scrutinize its relevant implication. Genomic DNA was extracted from blood samples of patients with a normal ovarian response (NOR) or with OHSS. Samples were analyzed to detect polymorphisms MTHFR rs1801131, MTHFR rs1801133, AMHR2 rs2002555, LHCGR rs2293275, PGR rs10895068, and SERPINE1 rs1799889. Variations of MTHFR, AMHR2, LHCGR, and PGR genes were significantly associated with the developing OHSS. After correction for multiple analysis, this difference was not evident for PGR genotypes. The polymorphic alleles of MTHFR (rs1801131 C‐allele and rs1801133 T‐allele), AMHR2 (rs2002555 G‐allele), and LHCGR (rs2293275 G‐allele) were significantly more prevalent among patients with OHSS compared to those in the NOR group. In contrast, the minor allele of PGR single‐nucleotide polymorphism (SNP) (rs10895068, A‐allele) was more prominent among patients with a NOR than those with OHSS. No significant difference was observed in genotypes or alleles of SERPINE1 rs1799889. The observations indicated that the minor alleles of MTHFR, AMHR2, and LHCGR genes could be considered an independent risk factor in susceptibility to OHSS. Nevertheless, polymorphic allele in the PGR rs10895068 SNP contributes to preventing OHSS occurrence. Therefore, it can be argued that these genes have a significant impact on OHSS. Abstract : The polymorphisms of MTHFR, AMHR2, and LCGHR genes could promote the risk in susceptibility to ovarian hyperstimulation syndrome (OHSS), which highlight the impact of these genes and their polymorphisms on OHSS and could potentially provide a genetic profile, which may help to adjust an optimized protocol for ovarian stimulation before the in vitro fertilization (IVF) procedure in different individuals, especially in patients who are genetically predisposed to high responses to controlled ovarian stimulation (COS) or developed OHSS.