A Novel Approach to Enhance the Urease Activity of Sporosarcina pasteurii and its Application on Microbial-Induced Calcium Carbonate Precipitation for Sand. Issue 9 (21st October 2019)

• Record Type: Journal Article
• Authors: Zhao, Yang; Xiao, Zhiyang; Lv, Jie; Shen, Wanqing; Xu, Rongchao
• Is Part Of: Geomicrobiology journal. Volume 36:Issue 9(2019)
• Page Start: 819
• Page End: 825
• Publication Date: 2019-10-21
• Subjects: Sporosarcina pasteurii -- microbial-induced calcium carbonate precipitation -- urease; Geomicrobiology -- Periodicals; Biogeochemistry -- Periodicals; 579
• DOI: 10.1080/01490451.2019.1631911
• Languages: English
• ISSNs: 0149-0451

Abstract

Abstract: Urease is involved in the formation of carbonate sediments by microbial-induced calcium carbonate precipitation (MICP), and Sporosarcina pasteurii used extensively in this technique owing to its high urease production. In this study, a simple two-step culture method with the appropriate medium was developed to enhance the urease activity of S. pasteurii . Urea played an important role in the culture process, particularly during the pre-cultivation step and the newly developed method improved both urease activity and specific urease activity. Furthermore, the increase in urease activity by MICP resulted in increased production of calcium carbonate and better strength of bio-cemented sand.