Analysis for microbial denitrification and antibiotic resistance during anaerobic digestion of cattle manure containing antibiotic. (November 2019)

• Record Type: Journal Article
• Authors: Zhang, Kaiyu; Gu, Jie; Wang, Xiaojuan; Zhang, Xin; Hu, Ting; Zhao, Wenya
• Is Part Of: Bioresource technology. Volume 291(2019)
• Publication Date: 2019-11
• Subjects: Anaerobic digestion -- Antibiotic -- Denitrifying gene -- Network analysis -- Nitrous oxide; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121803
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Antibiotic caused greater losses of nitrogen during anaerobic digestion. Different response of denitrification genes to antibiotic possibly due to ARGs. Denitrification genes and ARGs could be located in same potential host bacteria. intI1 important for horizontal transfer of denitrification genes and ARGs. Abstract: This study investigated the effects of tylosin (0, 10, and 100 mg/kg dry weight) on the denitrification genes and microbial community during the anaerobic digestion of cattle manure. N2 emissions were reduced and N2 O emissions were increased by 10 mg/kg tylosin. Adding 100 mg/kg tylosin increased the emission of both N2 O and N2 . The different responses of denitrifying bacteria and genes to tylosin may have been due to the presence of antibiotic resistance genes (ARGs). Network analysis indicated that denitrification genes and ARGs had the same potential host bacteria. intI1 was more important for the horizontal transfer of denitrification genes and ARGs during anaerobic digestion than intI2 . The anaerobic digestion of manure containing tylosin may increase nitrogen losses and the associated ecological risk.