Isolation, characterization, and identification of potential Diuron-degrading bacteria from surface sediments of Port Klang, Malaysia. (February 2018)

• Record Type: Journal Article
• Authors: Hanapiah, Munirah; Zulkifli, Syaizwan Zahmir; Mustafa, Muskhazli; Mohamat-Yusuff, Ferdaus; Ismail, Ahmad
• Is Part Of: Marine pollution bulletin. Volume 127(2018)
• Page Start: 453
• Page End: 457
• Publication Date: 2018-02
• Subjects: Diuron -- Antifouling -- Biodegradation -- Bacteria -- Marine; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2017.12.015
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Diuron is an alternative biocide suggested to replace organotin in formulating antifouling paints to be applied on water-going vessels hull. However, it is potentially harmful to various non-targeted marine organisms due to its toxic properties. Present study aimed to isolate, screen and identify the potential of Diuron-degrading bacteria collected from the marine sediments of Port Klang, Malaysia. Preliminary screening was conducted by exposing isolated bacteria to 430 ng/L (background level), followed by 600 ng/L and 1000 ng/L of Diuron concentrations. Nine bacteria colonies survived the exposure of the above concentrations. However, only two strains can tolerate to survive up to 1000 μg/L, which were then characterised and identified using phenotypic tests and the standard 16S rRNA molecular identification. The strains were identified as Comamonas jiangduensis SZZ 10 and Bacillus aerius SZZ 19 (GenBank accession numbers: KU942479 and KU942480, respectively). Both strains have the potential of Diuron biodegradation for future use. Highlights: This study is the first to record indigenous bacteria isolated from a Diuron-contaminated port area in Malaysia. Comamonas jiangduensis SZZ 10 and Bacillus aerius SZZ 19 have been identified as the potential Diuron-degraders. These results pioneer the study of degrading Diuron in the Malaysian marine environment.