Synthesis of fluorescent molecularly imprinted nanoparticles for turn-on fluorescence assay using one-pot synthetic method and a preliminary microfluidic approach. (28th February 2018)

• Record Type: Journal Article
• Authors: Li, Qianjin; Jiang, Lingdong; Kamra, Tripta; Ye, Lei
• Is Part Of: Polymer. Volume 138(2018)
• Page Start: 352
• Page End: 358
• Publication Date: 2018-02-28
• Subjects: Molecularly imprinted polymer -- One-pot synthesis -- Fluorescence resonance energy transfer; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2018.01.086
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: Fluorescent molecularly imprinted polymers (MIPs) have received considerable attention for their promising applications in sensing, imaging and diagnostics. Different synthetic methods have been reported to prepare fluorescent MIPs. Here, one-pot polymerization method was developed to synthesize fluorescent MIP nanoparticles (NPs) bearing a suitable fluorescent label as the fluorescence acceptor. The MIP NPs can emit strong fluorescence based on fluorescence resonance energy transfer (FRET) when they bind to analytes acting as the fluorescence donor. The one-pot synthetic method is straightforward, less time-consuming and easy to optimize the loading of fluorescence acceptor. Additionally, a preliminary microfluidic reactor was designed for preparing such fluorescent MIP NPs with more uniform size in a continuous process for the first time. The fluorescent MIP NPs can be used for selective detection of the analytical target in a FRET-based turn-on fluorescence assay which is easy to carry out with no tedious separation steps. Graphical abstract: Molecularly imprinted polymer nanoparticles bearing a suitable fluorescence acceptor were synthesized by precipitation polymerization in one-pot as well as in a continuous microfluidic reactor. The imprinted nanoparticles emit strong fluoresce when they bind to an analytical target. Highlights: One-pot synthesis of fluorescent molecularly imprinted polymer nanoparticles. Continuous synthesis of polymer nanoparticles by precipitation polymerization in a microfluidic reactor. Turn-on fluorescence assay using fluorescent molecularly imprinted nanoparticles.