Effect of model root exudate on denitrifier community dynamics and activity at different water-filled pore space levels in a fertilised soil. (May 2018)

• Record Type: Journal Article
• Authors: Langarica-Fuentes, Adrian; Manrubia, Marta; Giles, Madeline E.; Mitchell, Susan; Daniell, Tim J.
• Is Part Of: Soil biology and biochemistry. Volume 120(2018)
• Page Start: 70
• Page End: 79
• Publication Date: 2018-05
• Subjects: Denitrification -- Root exudate addition -- Microbial community dynamics -- Nitrous oxide emissions -- nirK -- nirS -- nosZ; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2018.01.034
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Although a "rhizosphere effect" on denitrification rates has been established, a clear understanding of the effects of exudate addition on denitrifier community dynamics remains elusive. A microcosm experiment was designed to explore the interaction between exudate addition and soil moisture on community dynamics and denitrification rates. Artificial root exudate at 5 different carbon concentrations was added daily to soil microcosms at contrasting target WFPS (50, 70 and 90%). After a 7-day period, total denitrification and N2 O emission rates were measured and community dynamics assessed using molecular methods. The response of denitrifier genes to exudate addition was different, with nirS and nos Z-I showing a stronger effect than nirK and nos Z-II. Distinct community structures were observed for nirS and nosZ -I at 90% target WFPS when compared to 50% and 70%. Nir S denitrifier population size showed a ca. 5-Fold increase in gene copy number at 90% WFPS when exudate was added at the highest C input. Significant total denitrification and N2 O emission rates were observed only at 90% WFPS, which increased with C input. Our study improves the understanding of the complex interaction between microbial communities, the abiotic environment and process rates which can inform management practices aimed at increasing complete denitrification and controlling greenhouse gas production from agriculture. Highlights: Controlled microcosms used to assess exudate C x WFPS effects on denitrification. The response of different denitrifier communities ( nirK, nirS, nosZ ) to C is distinct. nirS and nosZ-I community size and structure strongly affected by carbon. WFPS is a key factor modulating denitrifier community changes via C addition. Higher WFPS and C concentrations resulted in greater denitrification rates.