An RNA-based approach to sequence the mitogenome of Hypoptopoma incognitum (Siluriformes: Loricariidae). (2nd September 2016)

• Record Type: Journal Article
• Authors: Moreira, Daniel Andrade; Magalhães, Maithê G. P.; de Andrade, Paula C. C.; Furtado, Carolina; Val, Adalberto L.; Parente, Thiago Estevam
• Is Part Of: Mitochondrial DNA. Volume 27:Number 5(2016)
• Page Start: 3784
• Page End: 3786
• Publication Date: 2016-09-02
• Subjects: Fish -- heteroplasmy -- mtRNA -- RNA-Seq -- transcriptome
• DOI: 10.3109/19401736.2015.1079903
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: Hypoptopoma incognitum is a fish of the fifth most species-rich family of vertebrates and abundant in rivers from the Brazilian Amazon. Only two species of Loricariidae fish have their complete mitogenomes sequence deposited in the Genbank. An innovative RNA-based approach was used to assemble the complete mitogenome of H. incognitum with an average coverage depth of 5292×. The typical vertebrate mitochondrial features were found; 22 tRNA genes, two rRNA genes, 13 protein-coding genes, and a non-coding control region. Moreover, the use of this approach allowed the measurement of mtRNA expression levels, the punctuation pattern of editing, and the detection of heteroplasmies.