The complete mitochondrial genome of Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae). (2nd September 2016)

• Record Type: Journal Article
• Authors: Sun, Tian Yi; Li, Lei; Xin, Tianrong; Wang, Yayu; Xia, Bin
• Is Part Of: Mitochondrial DNA. Volume 27:Number 5(2016)
• Page Start: 3676
• Page End: 3677
• Publication Date: 2016-09-02
• Subjects: Cryptolestes ferrugineus -- mitochondrial genome -- phylogenetic relationships
• DOI: 10.3109/19401736.2015.1079851
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: We determined the complete mitochondrial genome (mitogenome) sequence of Cryptolestes ferrugineus (GenBank accession number KT182067) by the long PCR and primer walking method. The mitochondrial genome is a typical circular DNA molecule of 15 511 bp in length, and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region (D-loop). The order of 37 genes was typical of insect mitochondrial DNA sequences described to date. The base composition of the genome is A (39.17%), T (37.24%), C (14.22%), and G (9.37%) with an A + T-rich hallmark as that of other invertebrate mitochondrial genomes. All protein-coding genes start with ATN codon and terminate with the stop codon T (AA) or TAG. The A + T-rich region is located between 12S rRNA and tRNA Ile . In this study, the phylogenetic relationships of Coleoptera species were constructed based on the nucleotides sequences of 13 PCGs of mitogenomes. The molecular-based phylogeny supported the traditional morphological classification on relationships within Coleoptera species.