The anti-estrogenicity of chronic exposure to semicarbazide in female Japanese flounders (Paralichthys olivaceus), and its potential mechanisms. Issue 2 (April 2018)

• Record Type: Journal Article
• Authors: Yue, Zonghao; Yu, Miao; Zhao, Haifeng; Wang, Jun; Zhang, Xiaona; Tian, Hua; Wang, Wei; Ru, Shaoguo
• Is Part Of: Marine pollution bulletin. Volume 129:Issue 2(2018)
• Page Start: 806
• Page End: 812
• Publication Date: 2018-04
• Subjects: SMC semicarbazide -- HPG axis hypothalamic-pituitary-gonadal axis -- GABA gamma-aminobutyric acid -- kiss kisspeptin -- gpr54 g-protein-coupled receptor 54 -- E2 17β-estradiol -- T testosterone -- ERs estrogen receptors -- VTG vitellogenin -- CHG choriogenin -- GnRH gonadotropin-releasing hormone -- GtHs gonadotropic hormones -- FSH follicle-stimulating hormone -- LH luteinizing hormone -- sbGnRH seabream gonadotropin-releasing hormone -- cGnRH II chicken gonadotropin-releasing hormone II -- sGnRH salmon gonadotropin-releasing hormone -- fshβ follicle-stimulating hormone beta subunit -- lhβ luteinizing hormone beta subunit -- fshr follicle-stimulating hormone receptor -- lhr luteinizing hormone receptor -- GAD glutamic acid decarboxylase -- dph days post hatching -- MS-222 3-aminobenzoic acid ethyl ester -- ELISA enzyme-linked immunosorbent assay -- ELS early life stages -- EDCs endocrine disrupting chemicals -- star steroidogenic acute regulatory protein -- cyp17 17α-hydroxylase -- 17β-hsd 17β-hydroxysteroid dehydrogenase -- cyp19a cytochrome P450 19A -- 3β-hsd 3β-hydroxysteroid dehydrogenase -- cyp11a1 cytochrome P450 11A1; Semicarbazide -- Japanese flounder -- Hypothalamic-pituitary-gonadal axis -- Gamma-aminobutyric acid -- Kisspeptin; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2017.10.081
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: This study investigated the anti-estrogenic effects of chronic exposure to a new marine pollutant, semicarbazide (SMC; 1, 10, and 100 μg/L), in female Paralichthys olivaceus, as well as the associated mechanism. After 130 days of exposure, plasma 17β-estradiol and testosterone concentrations, and hepatic estrogen receptors, vitellogenin, and choriogenin mRNA levels decreased significantly in SMC-exposed groups. Moreover, down-regulation of genes in the hypothalamic-pituitary-gonadal axis, including gonadotropin-releasing hormone, gonadotropic hormones and their receptors, the steroidogenic acute regulatory protein, 17α-hydroxylase, 17β-hydroxysteroid dehydrogenase, and cytochrome P450 19A, was observed after SMC exposure. Furthermore, the kisspeptin/g protein-coupled receptor 54 (kiss/gpr54) system and gamma-aminobutyric acid-ergic (GABAergic) system were also affected by SMC: SMC significantly down-regulated mRNA expression of kiss2, gpr54, and the GABA synthesis enzyme gad67 . Our results demonstrated for the first time that environmentally relevant concentrations of SMC exerted anti-estrogenicity in female flounders, providing theoretical support for ecological risk assessments of SMC in marine environments. Highlights: Semicarbazide (SMC) reduces plasma E2 and T levels in female flounders. SMC inhibits hepatic ERs, VTG, and CHG mRNA levels in female flounders. HPG-axis, GABAergic, and kiss/gpr54 genes affect SMC-mediated E2 decreases. Environmentally relevant SMC concentrations have anti-estrogenic effects.