The isolation, structural characterization and anti-osteosarcoma activity of a water soluble polysaccharide from Agrimonia pilosa. (1st May 2018)

• Record Type: Journal Article
• Authors: Huang, Wei; Deng, Huan; Jin, Shengyang; Ma, Xuan; Zha, Kun; Xie, Mao
• Is Part Of: Carbohydrate polymers. Volume 187(2018)
• Page Start: 19
• Page End: 25
• Publication Date: 2018-05-01
• Subjects: Agrimonia pilosa -- Polysaccahride -- Osteosarcoma -- Apoptosis -- Mitochondria; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.01.047
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A homogenous polysaccharide (APP) is isolated from Agrimonia pilosa . APP induces apoptosis of human osteosarcoma U-2 OS cells in vitro. APP suppresses the growth of U-2 OS xenograft tumors in vivo. APP-induced apoptosis is via the activation of a mitochondrial pathway. Abstract: A homogenous polysaccharide (APP), with a molecular weight of 120 kDa, was isolated from the dried aerial parts of Agrimonia pilosa . Gas chromatography (GC) and GC–MS analysis revealed that APP has a backbone of 1, 3-linked Glcp and 1, 3, 6-linked Glc p, and branched with 1-linked Glc p terminal along the main chain in a relative ratio of 2:1:1. We investigated the response of human osteosarcoma U-2 OS cells to APP treatment. MTT result showed that APP significantly inhibited cell viability in a concentration dependent manner via induction of apoptotic death in U-2 OS cells, as determined by annexin V/propidium iodide (PI) staining. Western blot analysis also indicated that APP CRA increased in Bax/Bcl-2 ratios by up-regulating Bax expression and triggered the release of cytochrome c from mitochondria into the cytoplasm. Moreover, APP supplement induced the activation of caspase-3, and −9, but not caspase-8 in U-2 OS cells. Likewise, APP administration significantly suppressed tumor growth in BALB/C nude mice bearing U-2 OS xenograft tumors. All these results indicate that APP-induced apoptosis is associated with the activation of a caspase-3-mediated mitochondrial pathway.