Photo-triggered recognition between host and guest compounds in a giant vesicle encapsulating photo-pierceable vesicles. (January 2018)

• Record Type: Journal Article
• Authors: Suzuki, Kentaro; Machida, Kotaro; Yamaguchi, Kazuo; Sugawara, Tadashi
• Is Part Of: Chemistry and physics of lipids. Volume 210(2018)
• Page Start: 70
• Page End: 75
• Publication Date: 2018-01
• Subjects: Giant vesicle -- Vesosome -- dsDNA -- Fluorescence probe -- Caged compound -- SYBR Green I -- Photo-triggered recognition -- Photo-pierceable GV; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipids; Periodicals; Electronic journals; 547.77
• DOI: 10.1016/j.chemphyslip.2017.11.008
• Languages: English
• ISSNs: 0009-3084

Abstract

Highlights: A method for preparing a GV-in-GV which comprises a photo-resistant outer GV and a photo-pierceable inner GV has been developed. The membrane composition of photo-pierceable GV was optimized by using single GV experiment. The photo-triggered recognition in a GV-in-GV occurred between the fluorescence probe and the released dsDNA from the inner GV. Abstract: Here, we used centrifugal precipitation to construct a giant vesicle (GV) encapsulating smaller giant vesicles (GV-in-GV) which comprises a photo-resistant outer GV and a photo-pierceable inner GV; the outer GV contained a fluorescent probe (SYBR Green I) in its inner water pool, and the inner GV contained double-stranded DNA (dsDNA) in its inner water pool. The phospholipid membrane of the inner GV was made photo-pierceable by inclusion of ca. 15 mol% of a caged phospholipid in its membrane. Immediately after exposure of the GV-in-GVs to UV irradiation, strong fluorescence was detected in the inner water pool of the outer GV, indicating that dsDNA had been released from the inner GV and had complexed with the fluorescent probe. These dynamics can be recognized as a macroscopic representation of the molecular level function of a caged compound.