Relative survival potential of platelets is associated with platelet CXCR4/CXCR7 surface exposure and functional recovery following STEMI. (November 2018)

• Record Type: Journal Article
• Authors: Rath, Dominik; Chatterjee, Madhumita; Meyer, Lennart; Tekath, Nina; Olma, Carolin; Krumm, Patrick; Adams, Constantin; Borst, Oliver; Müller, Karin; Droppa, Michal; Nikolaou, Konstantin; Riethmüller, Joachim; Gawaz, Meinrad; Geisler, Tobias
• Is Part Of: Atherosclerosis. Volume 278(2018)
• Page Start: 269
• Page End: 277
• Publication Date: 2018-11
• Subjects: CXCR4 -- CXCR7 -- Survival -- STEMI -- Platelets; Arteriosclerosis -- Periodicals; Electronic journals; 616.136
• DOI: 10.1016/j.atherosclerosis.2018.10.008
• Languages: English
• ISSNs: 0021-9150

Abstract

Abstract: Background and aims: Platelets are critically involved in tissue repair and regeneration, which depend on their inflammatory properties and survival. SDF-1 ligates to CXCR4 and CXCR7 and contributes to the regulation of platelet survival. Platelet CXCR4/CXCR7 are involved in myocardial regeneration after infarction and are associated with outcomes in patients with symptomatic coronary artery disease. This study investigates the CXCR4/CXCR7 platelet survival axis ex vivo . Methods: 87 patients with ST-segment elevation myocardial infarction (STEMI) were included and analyzed for platelet surface exposure of CXCR4, CXCR7, Annexin V binding and tetramethylrhodamine ethyl ester (TMRE) response. Serum of 38 patients was analyzed for FasL, TNFα, TNF RI, TNF RII and TRAIL with Bioplex ® . The majority of patients received sequential cardiac MRI (intrahospital, 6-month follow-up). Results: We found a strong and positive correlation between surface exposure of CXCR4 and CXCR7 (ρ = 0.856, p <0.001). Relative survival potential correlated significantly with both platelet surface exposure of CXCR4 and CXCR7 (ρ = 0.365, p = 0.019; ρ = 0.417, p = 0.006) and furthermore with improvement of myocardial left ventricular ejection fraction (LVEF) (ρ = 0.490, p = 0.013). High relative survival potential showed significantly higher levels for both CXCR4 and CXCR7 surface exposure (MFI 87.3 vs. 69.0, p = 0.037; MFI 71.4 vs. 59.3, p = 0.045). We found a significant change in absolute LVEF% over the course of 6 months in patients with high CXCR7 platelet surface exposure (LVEF% 44.3 vs. 60.0, p ≤0.001). Conclusions: Platelet survival is associated with platelet surface exposure of CXCR4 and CXCR7 in STEMI patients and contributes to functional recovery after STEMI. Highlights: In a homogeneous STEMI collective, platelet expression of CXCR4 and CXCR7 is highly correlated with each other. High platelet CXCR7 surface exposure is associated with improved functional recovery (LVEF%) in STEMI patients. Platelet survival is associated with platelet surface exposure levels of the pro-survival receptors CXCR4 and CXCR7. Platelet survival is associated with recovery of LVEF% after STEMI. Relative platelet survival potential might be upregulated in STEMI patients compared to those with stable CAD and healthy subjects.